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RNA quality


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3 replies to this topic

#1 Mar_Bio

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Posted 05 October 2010 - 10:22 AM

Hi,
I have some problems with my folmaldehyde gels which I run to assess RNA quality. I can see two distinct bands for 28S and 18S. However I can also see third faded band above the 28S one. Is my RNa degraded? I was wondering if someone else had this issue. Any suggestions, comments will be apreciated.

#2 Trof

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Posted 06 October 2010 - 08:10 AM

Do you have a picture?
If your band is above the 28S I assume it's bigger. It could be DNA contamination, RNA degradation would produce smaller fragments and visible decrase of 28S in comparison with 18S.

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#3 Mar_Bio

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Posted 06 October 2010 - 04:18 PM

Not sure how to attach files.can I send it to you by email? What would DNA contamination do to my RT-PCR results.
Thank you.

#4 Mar_Bio

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Posted 05 November 2010 - 03:24 PM

Do you have a picture?
If your band is above the 28S I assume it's bigger. It could be DNA contamination, RNA degradation would produce smaller fragments and visible decrase of 28S in comparison with 18S.

RNA gel.jpg




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