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Immunioprecipitation from Jurkat cells??


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#1 Injo

Injo

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Posted 03 October 2010 - 11:22 PM

Has anybody here used Jurkat cells and Protein G Dynabeads for immunoprecipitation? I get very strange results.

#2 Kristina @ Invitrogen Dyna

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Posted 11 October 2010 - 06:18 AM

Hi Injo

Dynabeads can be used for immunoprecipitation from any cell type, including Jurkat cells. I have done many IP studies on Jurkat cells with excellent results.
What kind of results are you seeing? Do you have background or low capture? What is your target protein and which antibody are you using?

I would be glad to help troubleshoot your IP.

Please feel free to write to me directly at eurotech@lifetech.com
Be sure to put 'Dynabeads' in the subject line for faster handling.

Kind regards

Kristina
Technical Support
Invitrogen Dynal




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