I am using Lipofectamine 2000 to transfect Pre-miRs from Ambion. However, recently I have been getting low cell viability when transfecting the Negative Control #1 (scrambled sequence) when comparing this with cells containing transfection agent-containing medium alone, no added pre-mirs.
Would there be any explanation for this?
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Low cell viability with transfection agent and negative control
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