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Low cell viability with transfection agent and negative control


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#1 Baars01

Baars01

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Posted 03 October 2010 - 01:35 PM

Hi,

I am using Lipofectamine 2000 to transfect Pre-miRs from Ambion. However, recently I have been getting low cell viability when transfecting the Negative Control #1 (scrambled sequence) when comparing this with cells containing transfection agent-containing medium alone, no added pre-mirs.

Would there be any explanation for this?

Thanks




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