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how to calculate serial dilutions using the hemocytometer


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#1 andrea764

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Posted 02 October 2010 - 11:02 AM

hey im in my final year in biomedical science but im still a bit lost when it comes to serial dilutions. In our lab the other day I got an average of 51 cells and our dilution factor was 1/100. I used 5 large squares so the cell volume was 50000. I carried out my calculation like this:
51x50000x100, however when i looked at the notes my dmonstrator had given me it said to divide by 100 not multiply to give a result of 25.5x10power of 3 (sorry i dont know how to do the power sign on this computer lol!).
However I needed to have 2x10power3 cells/ml as my final concentration, but im completely lost:(can someone pls help!!

andrea

#2 bob1

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Posted 02 October 2010 - 02:03 PM

See the attachment in this thread http://www.protocol-...5266#entry55266




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