Hello,
Please suggest me methods to detect apoptosis in tumor tissue slides as well as samples samples.
I did Tunnel staining but I am unable to find tunnel positive cells due to high background.
I will appreciate the help.
thank you.
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4 replies to this topic
#2
nightingale
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Posted 04 October 2010 - 12:15 PM
hello 
kindly read the attached article, hope you will find it beneficial.
advanes in protocols for detecting apoptosis.pdf 604.15K
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Best Wishes
kindly read the attached article, hope you will find it beneficial.
advanes in protocols for detecting apoptosis.pdf 604.15K
128 downloadsBest Wishes
" The more you learn, the more you realize how little you know ... "
#3
moljul
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Posted 12 October 2010 - 12:30 AM
check for the cleaved form of caspase 3!
#4
deep
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Posted 13 October 2010 - 02:25 PM
nightingale, on 04 October 2010 - 12:15 PM, said:
hello
kindly read the attached article, hope you will find it beneficial.
advanes in protocols for detecting apoptosis.pdf
Best Wishes
kindly read the attached article, hope you will find it beneficial.
advanes in protocols for detecting apoptosis.pdfBest Wishes
Thank you for tthe suggestion..
Deep
#5
Wiggum
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Posted 23 December 2010 - 07:19 AM
If epithelial cells, you can also try measuring cytokeratin 18 (ELISAs are available for few different methods)
