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please suggest methods to detect apoptosis


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4 replies to this topic

#1 deep

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Posted 01 October 2010 - 03:07 PM

Hello,
Please suggest me methods to detect apoptosis in tumor tissue slides as well as samples samples.
I did Tunnel staining but I am unable to find tunnel positive cells due to high background.
I will appreciate the help.
thank you.

#2 nightingale

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Posted 04 October 2010 - 12:15 PM

hello :)

kindly read the attached article, hope you will find it beneficial.

Attached File  advanes in protocols for detecting apoptosis.pdf   604.15KB   218 downloads

Best Wishes

" The more you learn, the more you realize how little you know ... "

#3 moljul

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Posted 12 October 2010 - 12:30 AM

check for the cleaved form of caspase 3!

#4 deep

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Posted 13 October 2010 - 02:25 PM

hello :)

kindly read the attached article, hope you will find it beneficial.

Attached File  advanes in protocols for detecting apoptosis.pdf   604.15KB   218 downloads

Best Wishes


Thank you for tthe suggestion..
Deep

#5 Wiggum

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Posted 23 December 2010 - 07:19 AM

If epithelial cells, you can also try measuring cytokeratin 18 (ELISAs are available for few different methods)




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