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How to amplify repetitive cDNAs regions

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#1 ana moreira

ana moreira


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Posted 01 October 2010 - 06:33 AM


I'm trying to clone for the first time a new gene in a fish specie by homology cloning. Comparing with other organisms I could see that the region I'm trying to clone is highly repetitive and codify to a conserved and characteristic domain of the protein.

So, I wanted to know if someone knows some technique that improve the PCR amplification in repetitive regions? I have heard something like add glycerol in the reaction...


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