Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to amplify repetitive cDNAs regions


  • Please log in to reply
No replies to this topic

#1 ana moreira

ana moreira

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 01 October 2010 - 06:33 AM

Hi!

I'm trying to clone for the first time a new gene in a fish specie by homology cloning. Comparing with other organisms I could see that the region I'm trying to clone is highly repetitive and codify to a conserved and characteristic domain of the protein.

So, I wanted to know if someone knows some technique that improve the PCR amplification in repetitive regions? I have heard something like add glycerol in the reaction...

Thanks!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.