Bisulfite conversion kits
Posted 01 October 2010 - 03:25 AM
I have been working with methylation studies but I can not obtain good results. I have used a bisulfite conversion kit from Qiagen, another for Ecogen and right now Invitrogen gave me a sample of the menthylcode bisulfite conversion kit.
I would like to open a discusion between those people that has used these kits or another ones to say what they think about (it works, it not, it degrades the DNA, it is needed highly purified DNA,...)
Everything you coment would be a help for me to decide what to do.
Thanks you very much
Posted 01 October 2010 - 04:47 AM
How were your DNA's prepared? what amount did you use for conversion and what controls you performed to assess that your conversion has failed?
Posted 01 October 2010 - 06:12 AM
Posted 04 October 2010 - 01:26 AM
I do not say that the conversion has failed, but the most part of DNA is degraded obtaining only 1/3 or less quantity of the DNA used for the conversion.
Probably I am doing something in the incorrect way but I can not found what it is. I will be really greatful if you give me some advice.
Posted 05 October 2010 - 05:03 PM
The next question for you now is, how are you determining your bisulphite conversion has worked or not? Nanodrop? PCR? or other means?
Posted 06 October 2010 - 12:59 AM
Posted 06 October 2010 - 07:01 AM