Hi all!
I have a question related to trypsin/EDTA preparation. I have 100ml comercial trypsin solution (x1) and I have EDTA disodium in powder. Is it correct to add directly 20gr of EDTA (0.53mM) to the 100ml trypsin solution, filter and store in aliquots???.... It will disolve correctly?? I have been looking for methods to do that but I have found nothing.
thanks so much!!
Submit your paper to J Biol Methods today!
1 reply to this topic
#2
Adrian K
-
- Global Moderators
-
- 705 posts
Legendary Graduate Beggar
27
Excellent
Excellent
Posted 02 October 2010 - 04:07 AM
Hi all!
I have a question related to trypsin/EDTA preparation. I have 100ml comercial trypsin solution (x1) and I have EDTA disodium in powder. Is it correct to add directly 20gr of EDTA (0.53mM) to the 100ml trypsin solution, filter and store in aliquots???.... It will disolve correctly?? I have been looking for methods to do that but I have found nothing.
thanks so much!!
Hmm... I'm not sure for such a small amount of EDTA, but based on my experience when I prepare a more concentrated EDTA, the pH is crucial for the solubility of EDTA.
Edited by adrian kohsf, 02 October 2010 - 04:08 AM.
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
