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Apoptosis induction


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5 replies to this topic

#1 Stone

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Posted 30 September 2010 - 03:02 PM

Hi everyone!

I need to use a positive control for apoptosis in vero and mcf-7 cells. What do you recommend me?

Thanks

#2 bob1

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Posted 30 September 2010 - 04:13 PM

UV treatment

#3 Stone

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Posted 21 October 2010 - 06:26 AM

Ok!

For how much time?

Thanks!

#4 bob1

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Posted 21 October 2010 - 03:09 PM

You will need to titrate that for yourself as it will depend on the intensity of your UV source, the wavelengths used and the cell types. 50-100 mJ/cm2is usually a good starting point.

#5 bori

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Posted 29 December 2010 - 12:16 PM

You can induce apoptosis using staurosporine.

#6 scolix

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Posted 30 December 2010 - 09:47 AM

You can induce apoptosis using staurosporine.


I agree with bori. Its best to use staurosporine or something similar.

Staurosporine: 1 uM, 6-12 hrs, usually kills 50% cells.




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