Hi everyone!
I need to use a positive control for apoptosis in vero and mcf-7 cells. What do you recommend me?
Thanks
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5 replies to this topic
#2
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Hmmm, I think it's working
Posted 30 September 2010 - 04:13 PM
UV treatment
#4
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Hmmm, I think it's working
Posted 21 October 2010 - 03:09 PM
You will need to titrate that for yourself as it will depend on the intensity of your UV source, the wavelengths used and the cell types. 50-100 mJ/cm2is usually a good starting point.
#5
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Posted 29 December 2010 - 12:16 PM
You can induce apoptosis using staurosporine.
#6
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a student
Posted 30 December 2010 - 09:47 AM
bori, on 29 December 2010 - 12:16 PM, said:
You can induce apoptosis using staurosporine.
I agree with bori. Its best to use staurosporine or something similar.
Staurosporine: 1 uM, 6-12 hrs, usually kills 50% cells.
