Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Need pH 6 Cell culture media

  • Please log in to reply
1 reply to this topic

#1 LJay



  • Members
  • Pip
  • 1 posts

Posted 30 September 2010 - 01:39 PM


I am currently using DMEM + FBS + Pen strep for my cell line. I would like to bring the media down to a pH of 6. Everytime I bring the pH down, the CO2 in the incubator combined with the sodium bicarbonate in the media bring the pH right back up within a half hour. I have tried bringing the pH down to 4 then back up to 6, thinking I may have saturated the buffer however that is not the case. In the literature there are many instances in which media of pH 6 is used, without any mention of how it is prepared. If you have any suggestions please let me know!


#2 protolder



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 293 posts

Posted 30 September 2010 - 09:53 PM

Hola, you could buy the powdered medium and prepare it at the wish pH, and sterilize filtering by 0.22um.Sigma has it, and in its information comes how to made it. Good luck

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.