I am currently using DMEM + FBS + Pen strep for my cell line. I would like to bring the media down to a pH of 6. Everytime I bring the pH down, the CO2 in the incubator combined with the sodium bicarbonate in the media bring the pH right back up within a half hour. I have tried bringing the pH down to 4 then back up to 6, thinking I may have saturated the buffer however that is not the case. In the literature there are many instances in which media of pH 6 is used, without any mention of how it is prepared. If you have any suggestions please let me know!
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Need pH 6 Cell culture media
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