2 replies to this topic

[soymilk14]

i just want to ask how to estimate the size of my protein? how to estimate a 500 bp construct to kilodalton? thank you...

[K.B.]

If you know the sequence you can use online tools to translate it into amino acids and then use the amino acid sequence to calculate MW.

[shivasankari]

3 bases = 1 amino acid = 110da(approximately)
50 bases = 19 amino acids (app) = 110 X 19 Daltons

cheers

Shiva