microdexter, on 01 October 2010 - 03:57 PM, said:
The high acrylamide percentage is very likely the cause in my case because the phenomenon was much less severe when I used to use 8%-16% gels.
Well my acrylamide percentage can't go any lower, any suggestions?
i've worked with high percentage gels, as well.
first, make sure your plates are as clean as possible, don't give the gel a reason to pull off the plate (also, make sure they are not warped).
then, make sure the gel is completely polymerized and does not already display the air tracks.
finally, be careful when handling the plates. make sure you don't push or slide the plates apart, especially when mounting on the apparatus.
when you run the gel, don't let it heat up. if necessary, let it take longer to run.