Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

GST fusion rotein


  • Please log in to reply
1 reply to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 28 November 2001 - 10:00 PM

Hi, I tries to elute the GST-fusion rotein from the Glutathione beads. It does not work well for me, even though I used freshly made elution buffer. What is the alternative way to do that?

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 29 November 2001 - 10:00 PM

Hi Rui,

I'm using GST fusion protein as well and to elute the proteins from the Glutathion beads: Buffer: 10mM Tris/HCl pH 7.5, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 5mM Glutathion. After adding the glutathion, readjust the pH to 8 with solid Tris. You can try to let the column sit in elution buffer 10 mins before collecting the fractions. Usually, I have a good rate of recuperation.

Isabelle





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.