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[anonymous]

Hi, I tries to elute the GST-fusion rotein from the Glutathione beads.  It does not work well for me, even though I used freshly made elution buffer.  What is the alternative way to do that?

[anonymous]

Hi Rui,

I'm using GST fusion protein as well and to elute the proteins from the Glutathion beads:  Buffer:  10mM Tris/HCl pH 7.5, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 5mM Glutathion.  After adding the glutathion, readjust the pH to 8 with solid Tris.  You can try to let the column sit in elution buffer 10 mins before collecting the fractions.  Usually, I have a good rate of recuperation.

Isabelle