establishment of stable cell line using hek293t
Posted 29 September 2010 - 01:35 AM
I am going to use hek293t cells for expression of some proteins, I know hek293t cells are already transfected with TAg of sv40 and my expression plasmid has sv40 origion of replication, I read some articles that say sv40 containing plasmids can replicate in hek293t cells so can I use hek293t cells for establishment of stable cell lines using this plasmid or this cell line is only used for transient expression?
Posted 29 September 2010 - 07:41 AM
Posted 01 October 2010 - 01:12 AM