Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to homogenise tissue in buffer?


  • Please log in to reply
2 replies to this topic

#1 hianghao

hianghao

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 111 posts
0
Neutral

Posted 28 September 2010 - 11:58 PM

I grind 30mg mosquitoes (~15-20 individuals) in a 1.5ml tube with a micropestle after adding in Liquid nitrogen and continue to grind after adding buffer. Does this considered homogenising? Or i'll had to use syringe?

If i am to use a syringe, how do i load the solution into it? By taking it through the tip? Loading with a pipette? How does using syringe differ from using a pipette to mix the solution?

There is some sort of automater tissue grinder/homogeniser (i am not sure whether it is a rotor stator) in my lab. If i use that to grind my 1st sample, how should i wash it for my subsequent sample? Can i use ethanol?

Edited by hianghao, 29 September 2010 - 01:13 AM.


#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,811 posts
136
Excellent

Posted 29 September 2010 - 08:18 AM

grinding in a mortar and pestle (1.5 ml tube and micropestle) is considered homogenization (for the purpose of extraction).
talent does what it can
genius does what it must
i do what i get paid to do

#3 hianghao

hianghao

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 111 posts
0
Neutral

Posted 29 September 2010 - 04:15 PM

grinding in a mortar and pestle (1.5 ml tube and micropestle) is considered homogenization (for the purpose of extraction).


Thanks for the reply! :rolleyes:




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.