2 replies to this topic

[hianghao]

I grind 30mg mosquitoes (~15-20 individuals) in a 1.5ml tube with a micropestle after adding in Liquid nitrogen and continue to grind after adding buffer. Does this considered homogenising? Or i'll had to use syringe?

If i am to use a syringe, how do i load the solution into it? By taking it through the tip? Loading with a pipette? How does using syringe differ from using a pipette to mix the solution?

There is some sort of automater tissue grinder/homogeniser (i am not sure whether it is a rotor stator) in my lab. If i use that to grind my 1st sample, how should i wash it for my subsequent sample? Can i use ethanol?

Edited by hianghao, 29 September 2010 - 01:13 AM.

[mdfenko]

grinding in a mortar and pestle (1.5 ml tube and micropestle) is considered homogenization (for the purpose of extraction).

[hianghao]

mdfenko, on 29 September 2010 - 08:18 AM, said:

grinding in a mortar and pestle (1.5 ml tube and micropestle) is considered homogenization (for the purpose of extraction).

Thanks for the reply!