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cloning more than 1 gene into vector if no IRES available


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#1 Fluorescent

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Posted 28 September 2010 - 02:30 PM

Hi there,everyone :)

I want to clone 2 genes into a vector to use in Xenopus laevis, and I am not sure that fusing them is a good idea. Has anyone used an IRES in xenopus? not much info about that out there. And if there is no IRES, how would you get 2 different genes expressed in 1 vector???

many many thanks, smarties!

#2 Rsm

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Posted 28 September 2010 - 10:31 PM

You could try a 2A peptide. Here's a reference: Development of 2A peptide-based strategies in the design of multicistronic vectors. PMID:15934839. And here they use it for Xenopus: Manipulating Heat Shock Factor-1 in Xenopus Tadpoles: Neuronal Tissues Are Refractory to Exogenous Expression. PMID:20405018.

Cheers,

rsm
I got soul, but I'm not a soldier

#3 Fluorescent

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Posted 29 September 2010 - 06:34 AM

Vow, that's a great advice, rsm!!! Thanks so much - and you even found a xenopus reference!!!! I will definitely try the 2A out.




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