2 replies to this topic

[Fluorescent]

Hi there,everyone

I want to clone 2 genes into a vector to use in Xenopus laevis, and I am not sure that fusing them is a good idea. Has anyone used an IRES in xenopus? not much info about that out there. And if there is no IRES, how would you get 2 different genes expressed in 1 vector???

many many thanks, smarties!

[Rsm]

You could try a 2A peptide. Here's a reference: Development of 2A peptide-based strategies in the design of multicistronic vectors. PMID:15934839. And here they use it for Xenopus: Manipulating Heat Shock Factor-1 in Xenopus Tadpoles: Neuronal Tissues Are Refractory to Exogenous Expression. PMID:20405018.

Cheers,

rsm

[Fluorescent]

Vow, that's a great advice, rsm!!! Thanks so much - and you even found a xenopus reference!!!! I will definitely try the 2A out.