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Cleaning RNase


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#1 Ban

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Posted 28 September 2010 - 09:42 AM

How do i clean out the RNase from my genomic DNA before restriction digestion. I just realised that the amount of RNase i added was not enough after runnibg a few AFLP gels. I am highly suspecting the amount of RNase i used. I also diluted the RNase very much.Is there a way of getting rid of this RNA without doing another extraction

#2 HomeBrew

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Posted 28 September 2010 - 11:46 AM

Do another RNAse digestion with the correct amount? Why do you need to remove the RNAse before restriction digestion?




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