Cell pellet frozen -80 then -20
Posted 28 September 2010 - 07:38 AM
Its my first time to post anything here and I hope I can get some help. My problem is that I had 12 different cell lines, snap frozen them and stored in -80C so later on I can extract protein for western blotting. However we moved labs from one city to another and the cell pellets were misplaced from -80C to -20C. Is it still possible to lyse them and extract my protein? given that my protein is in the plasma membrane.
Posted 28 September 2010 - 04:08 PM
Posted 29 September 2010 - 07:18 AM
Posted 04 October 2010 - 09:06 PM