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Cell pellet frozen -80 then -20


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4 replies to this topic

#1 selgebali

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Posted 28 September 2010 - 07:38 AM

Hey guys,
Its my first time to post anything here and I hope I can get some help. My problem is that I had 12 different cell lines, snap frozen them and stored in -80C so later on I can extract protein for western blotting. However we moved labs from one city to another and the cell pellets were misplaced from -80C to -20C. Is it still possible to lyse them and extract my protein? given that my protein is in the plasma membrane.
Thanks guys,

#2 bob1

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Posted 28 September 2010 - 04:08 PM

If they were frozen all the time they should be OK, though this will vary according to how long they have been stored and the presence of protease inhibitors.

#3 Don Boyce

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Posted 29 September 2010 - 07:18 AM

There can definitely be a difference between storage at -80 and -20, especially long term. But if it was only a brief period there probably won't be a problem. The only real way to find out is use them.

#4 why and how

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Posted 04 October 2010 - 09:06 PM

I think cells will be still functional and healthy in -20 freezer, provided not for a long time.

#5 selgebali

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Posted 05 October 2010 - 06:10 AM

Thanks guys. I really appreciate your help.




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