4 replies to this topic

[selgebali]

Hey guys,
Its my first time to post anything here and I hope I can get some help. My problem is that I had 12 different cell lines, snap frozen them and stored in -80C so later on I can extract protein for western blotting. However we moved labs from one city to another and the cell pellets were misplaced from -80C to -20C. Is it still possible to lyse them and extract my protein? given that my protein is in the plasma membrane.
Thanks guys,

[bob1]

If they were frozen all the time they should be OK, though this will vary according to how long they have been stored and the presence of protease inhibitors.

[Don Boyce]

There can definitely be a difference between storage at -80 and -20, especially long term. But if it was only a brief period there probably won't be a problem. The only real way to find out is use them.

[why and how]

I think cells will be still functional and healthy in -20 freezer, provided not for a long time.

[selgebali]

Thanks guys. I really appreciate your help.