Unfortunately I don't have something to say about yeast, but maybe I can bring your attention to yet another expression system ...
What I wanted to say is, that we had a similar problem some time ago and we went to express our protein in Drosophlia Schneider-2 cells in FCS-free medium.
Pros: Directly inducible by Cu2+ ions in the medium, since the GOI is under the regulation of a metallothioneine promotor, AND we got our protein secreted into the cell culture media, since our expression construct had a secretion-sequence, too. So, no need to crack or lyse cells, just a centrifugation, and there you have your precleared protein in suspension....
AND, if you're doing immunology with your protein, you have the guarantee that it's free of stimulatory prokaryotic factors such as bacterial DNA, or LPS etc.
Cons: You need some time to establish your stably transfected cell line, optimal induction times can vary from protein to protein (7 days in my case) AND your gene has to be expressed by DS-2 cells at all (codon bias, splicing etc.)
But when it works, it's a very fine thing indeed!
@katia: I think ragno was talking about expression E.coli and different induction temperatures...
Edited by jadefalcon, 27 July 2004 - 04:38 AM.