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Determine exon and intron for primer design

Started by Abiotic_Kabir, Sep 27 2010 05:37 AM

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#1 Abiotic_Kabir

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Posted 27 September 2010 - 05:37 AM

Hi,
How can I know the exon and intron in mRNA or cDNA before designing primer for qRT-PCR?
  
mRNA            join(<1..196,283..373,522..719,812..1046,1301..1628,
                     1745..1988,2146..2852,2930..>3069)
                     /gene="FRO1"
                     /product="iron reductase"

Are these the exon regions that I found in ncbi database?


Cheer!

#2 Curtis

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Posted 27 September 2010 - 09:08 AM

yes, they are. NCBI is correct

#3 Abiotic_Kabir

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Posted 28 September 2010 - 06:55 AM

thanks.
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