but after 14 days of transfection most of my cells are dying and i am unable to isolate any clone.
how do i continue at this rate?
how do i confirm whether the death is occurring due to the silencing of the desired gene, and this gene expression is indispensable to the survival of my cells due to which the cells are unable to survive or is the death occuring because the shRNA is somehow toxic to my cells.
is there any assay i can do to confirm this?
Edited by eurisko, 26 September 2010 - 10:52 AM.