Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

how to extract enzyme allinase from garlic

  • Please log in to reply
1 reply to this topic

#1 ikwana



  • Active Members
  • Pip
  • 27 posts

Posted 24 September 2010 - 09:12 PM

how to extract allinase from garlic? and how to purify it?

how to determine its concentration?
im going to incubate garlic in different temperature.. how is it to determine whether there is changes in the behaviour of enzyme..

allin will interact allinase to become allicin.. do i have to use allicin to observe the reaction of the allinase?

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,361 posts

Posted 28 September 2010 - 12:12 PM

this paper in plant physiology can give you details on purification and assay of alliinase (it was from onion but the procedure should work or you can use the references to get it for garlic):

a novel alliinase from onion roots

Edited by mdfenko, 28 September 2010 - 12:13 PM.

talent does what it can
genius does what it must
i used to do what i got paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.