Quantitative Western Imaging
Posted 23 September 2010 - 09:12 PM
Posted 23 September 2010 - 10:20 PM
Edited by Curtis, 23 September 2010 - 10:21 PM.
Posted 06 October 2010 - 11:01 AM
I know Quantity One (BioRad) will do this type of analysis. If you know someone that has a Gel Doc you might ask if you can analyze your images with their software. As far as over exposure, you could check your standards and if your upper concentrations flatten out then you are overexposed. Maybe take a couple exposures and use one that gives you a good curve.
Posted 07 October 2010 - 06:26 AM