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yeast spore elimination


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3 replies to this topic

#1 sara.r

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Posted 23 September 2010 - 11:34 AM

HI
I have a problem with contamination of my cell culture to yeasts, I double autoclaved samplers and pipets and start with new media but I still have the problem, so can yeast spores survive autoclaving? and can they remain in 70% alcohol that usually used for cleaning surfaces?


regards

#2 Adrian K

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Posted 23 September 2010 - 07:18 PM

Yes, some spores does survive ethanol and autoclaving...
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#3 sara.r

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Posted 23 September 2010 - 10:13 PM

Yes, some spores does survive ethanol and autoclaving...



So how can I get rid of them? do you have any suggestions?

#4 gebirgsziege

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Posted 23 September 2010 - 11:23 PM

Yeasts usually do not survive autoclaving. Some suggestions: Is there a possibility you carry them over with your cells or that your stock is contaminated? Lab bench and incubator are contamination free? Have you checked if your plastic ware is contaminated? And are you sure the concentration of your EtOH is ok? Is your autoclave working properly (check the logger)? Is anybody else using your chemicals, water etc.? Do you have an aircondition in the lab that might be the source of an airborne contamintation.
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