yeast spore elimination
Posted 23 September 2010 - 11:34 AM
I have a problem with contamination of my cell culture to yeasts, I double autoclaved samplers and pipets and start with new media but I still have the problem, so can yeast spores survive autoclaving? and can they remain in 70% alcohol that usually used for cleaning surfaces?
Posted 23 September 2010 - 07:18 PM
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
Posted 23 September 2010 - 10:13 PM
Yes, some spores does survive ethanol and autoclaving...
So how can I get rid of them? do you have any suggestions?
Posted 23 September 2010 - 11:23 PM