Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Precipitation of Native Protein


  • Please log in to reply
1 reply to this topic

#1 Robdom76

Robdom76

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 04 May 2002 - 12:48 AM

Hi,
Im purifying a His-tagged protein under native conditions via IMAC (Talon). While eluting (400mM Imidazole; 150mM KCl; 20mM Tris-HCl; 0.05% Triton X-100) I get precipitations and the concentration of protein in solution is quite low. Any ideas how to enhance solubility under native conditions (detergents, concentrations)?

Thanks,

robdom


#2 Sciad

Sciad

    member

  • Active Members
  • Pip
  • 12 posts
0
Neutral

Posted 03 July 2002 - 06:09 AM

If you can, check the isoelectric point (IEP) of your protein. If it stayed in solution before this step, it could be that the changed pH is the culprit. Proteins have lowest solubility at their IEP.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.