I am about to see gene expression in plant root. I need something to be clear since I am new in qRT-PCR.
1) Which housekeeping gene would be suitable as internal control for qRT-PCR?
2) I have to use rotor gene 3000 cycler. Which kit would suitable for this machine?
3) Is one-step qRT-PCR is easier than to-step?
4) I would see the expression of IRT1 gene. Someone used a pair of primer Arabidopsis IRT1 gene. can I use the same primer for other plant.
5) Which RNA isolation kit would be best for avoiding DNA contamination? Trizol or Qiagen?
Sorry for too many questions. I would be really grateful to have suggestions from you guys.