Recently, a friend of mine approached me for some technical help in tagging her osteosarcoma cells with GFP. One of her friends recommended her to use the Retro-X Universal Packaging System and pRetroQ-AcGFP-N1 cloning vector from Clontech for her. When I talked to her to understand her purpose her project and downstream applications, I felt she don't really need such an expensive system to do a simple GFP-tagging.
Her background is in tissue engineering and possess no idea how such work can be done. She just need her cell lines to be tagged with GFP so that she'd be able to view the cells under a florescence microscope. I'm thinking she might just need to transfect her cells with normal GFP-tagged vector, as I supposed she's doing this as a positive control. However, I realised that she actually need to inject these GFP-tagged cells into mice to track the movement of the cells and she's not doing any sort of gene expression work. As I ask around, it seems that she needs to create a stable cell line with GFP prior in injecting the cells into an animal system.
Now the question is, how can I go about in doing this? It seems to me that it's just more than just transfecting the GFP-vector into her cells. As I search around, most GFP vectors are cloning vectors and are quite expensive for such experiment (I think). I wonder is there some other vector/plasmids tagged with GFP for such work, or some other methods that's feasible.
Need advise for this.
Thank you very much
Edited by Katie Z, 22 September 2010 - 06:10 PM.