Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

expression


  • Please log in to reply
No replies to this topic

#1 Jhimli Dasgupta

Jhimli Dasgupta

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 18 July 2002 - 10:45 PM

I have tried the expression of a 20kD protein, cloned in pET3a in BL21 system. Bulk cultute is grown upto 0.5 OD at 600 nm and IPTG is added in 3 different conc. 0.1mM, 0.2mM and 0.3mM in 3 different sets and culture is collected after 1hr and 2hr induction. But I could not see the desired band in SDS-PAGE.    Please give me suggestion.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.