'Stringy' contamination of cells?
Posted 22 September 2010 - 07:14 AM
I did my PhD in microbiology and have been introduced to tissue culture relatively late in life, so I'm prone to worrying about whether the cells look 'right'. I'm currently maintaining a human osteosarcoma line (SAOS-2) and have noticed little stringy bits in the culture flask. The cells seem fine (viable, no pH changes, etc) but it does worry me a little. Others in my lab reckon it's FBS-related rubbish and that I shouldn't worry, but I'd like some corroboration.
Any help/comments would be appreciated!
Posted 22 September 2010 - 10:24 AM
Posted 23 September 2010 - 02:18 AM
I agree with madrius1 that what you are seeing are a fibre, hair or even possibly a piece of TC plastic that is left over from the manufacturing process. I am almost 100% sure that it is not a fungal contamination.
Hope this is reassurring
Posted 23 September 2010 - 02:32 AM