regenerate qiagen midi columns
Posted 22 September 2010 - 03:30 AM
I would like to reuse the midiprep columns qiagen (the ones that use QBT to equilibrate them first).
I didn't find any method available and I presume that if you know the material they are probably we should be able to regenerate them for a new preparation. I am sure I will use them only for the same plasmid extraction.
Many thanks and Best things
Posted 23 September 2010 - 02:43 AM
Posted 23 September 2010 - 02:55 AM
I will check the maxxbond.de website with a friend, translating the german language.
I tried to regenerate the spin columns (MIDI Qiagen) with:
- 10 mL ddH2O
- 4 mL QBT
and then used them with the original protocol for midiprep.
And the resulting pellet had a very good quality.
Clearly I used the column for the same plasmid purified previously.
I won't use the column for different plasmids.
I am storing the spin-column wet in ddH2O but I am thinking about a method to dry it before to store it, to avoid mould.
Best of luck
Posted 23 September 2010 - 09:27 AM