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Sequencing of receptor variants in tumor

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#1 dna_nerd



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Posted 21 September 2010 - 08:05 PM

Hello all, I have a prelim coming up where I need to present on methods out of my jurisdiction.

I would like to take samples of primary tumor and sequence two well-characterized receptors to look at genetic variation present in them.

How do I prep genomic DNA for sequencing, do I need next-generation sequencing (Illumina machine) or an older method, once I have sequences how do I line them up and look for patterns?

Any advice or direction is appreciated, cheers.

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