Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Sequencing of receptor variants in tumor


  • Please log in to reply
No replies to this topic

#1 dna_nerd

dna_nerd

    member

  • Active Members
  • Pip
  • 27 posts
0
Neutral

Posted 21 September 2010 - 08:05 PM

Hello all, I have a prelim coming up where I need to present on methods out of my jurisdiction.

I would like to take samples of primary tumor and sequence two well-characterized receptors to look at genetic variation present in them.

How do I prep genomic DNA for sequencing, do I need next-generation sequencing (Illumina machine) or an older method, once I have sequences how do I line them up and look for patterns?

Any advice or direction is appreciated, cheers.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.