Could somebody please help me in calculating the migration distance ofa restriction digest on paper and not by performing the actual laboratory experiment following the Agarose or polyacrylamide gel electrophoresis. Provided the applied voltage, concentration of agarose, fragment length, duration of gel run and the gel length is known.
This calculation of migration distance could be dependent upon the fragment length, ( I know this because I have the restiction map of the sequence to be digested assuming the enzymes used to restriciton digest the sequence will cut at all possible restriciton sites) and on the agarose concentration in the gel.
Please do me a reply b/c, now its hights of its need to resolve the matter.
Thank you in advance for any reply