Hi, I'm a bit confused as to why my RNA doesn't migrate to the same location when running denaturing agarose gel electrophoresis. Could it be because I need more buffer (the amount I use right now barely covers the top of the gel). The procedure I follow is similar to this http://www.ambion.co...pp/rna_gel.html
I'm not sure what's going on..any help is appreciated. Thanks.
RNA bands not migrating to same distance
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