I am going to do mRNA preparation from total RNA.
In doing this, what is the role of poly A (column)?
Anyone of experts in theories could explain it to me?
Please!!
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#2
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Brain on a stick
Posted 22 September 2010 - 07:46 AM
The kit may be called "poly A" but the column actually contains poly-T beads. A (adenine) is complementary to T (thymine).
If you look for the key features of mRNA you will find out why T polymers are used.
If you look for the key features of mRNA you will find out why T polymers are used.
Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.
#3
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Posted 22 September 2010 - 09:26 AM
Trof, on 22 September 2010 - 07:46 AM, said:
The kit may be called "poly A" but the column actually contains poly-T beads. A (adenine) is complementary to T (thymine).
If you look for the key features of mRNA you will find out why T polymers are used.
If you look for the key features of mRNA you will find out why T polymers are used.
Thanks a lot.
