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mRNA preparation from total RNA


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#1 doctormoon

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Posted 19 September 2010 - 12:49 PM

I am going to do mRNA preparation from total RNA.
In doing this, what is the role of poly A (column)?
Anyone of experts in theories could explain it to me?
Please!!

#2 Trof

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Posted 22 September 2010 - 07:46 AM

The kit may be called "poly A" but the column actually contains poly-T beads. A (adenine) is complementary to T (thymine).
If you look for the key features of mRNA you will find out why T polymers are used.

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#3 doctormoon

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Posted 22 September 2010 - 09:26 AM

The kit may be called "poly A" but the column actually contains poly-T beads. A (adenine) is complementary to T (thymine).
If you look for the key features of mRNA you will find out why T polymers are used.

Thanks a lot.




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