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Archiving cell lysates for future 2D analysis?

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#1 tellicherry



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Posted 28 February 2002 - 05:58 PM


We're undertaking the collection of a large number of cell samples for eventual proteomic analysis.  However, hundreds of samples will be collected over a period of three weeks and the 2D workup of these samples will take *many* months.

Does anyone have any insights or suggestions as to the best way to archive the cell samples so that degredation and modification will be minimized?  My first inclination is to lyse the harvested cells in a standard lysis buffer (8M urea, CHAPS, tris) and store them at -80.  However, I'm not sure that this is really the best.



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