Can anyone suggest a protocol for isolation and quantification of the B-1,3-glucanase enzyme from alnus glutinosa roots
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franck
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Posted 13 March 2002 - 01:23 AM
same pb, but i try to fond something in the mips web
http://www.mips.biochem.mpg.de in yeast in search tool the gene name is exg1. There is a lot of litterature and probably the method, but i isolate an isoform in using mutant with vanadate(!?). Indeed the form is cytoplasmic and it's the only protein that stay in the cell wall of my cells after treatement (verification by maldi-tof)
http://www.mips.biochem.mpg.de in yeast in search tool the gene name is exg1. There is a lot of litterature and probably the method, but i isolate an isoform in using mutant with vanadate(!?). Indeed the form is cytoplasmic and it's the only protein that stay in the cell wall of my cells after treatement (verification by maldi-tof)
