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Finding splicing factors specific to an RNA of interest.


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#1 ethan241

ethan241

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Posted 17 September 2010 - 10:55 AM

Are there any assays or kits that help one discover which splicing factors bind to certain RNAs?

Or could one make a bead with a specific RNA on it, then mix a nuclear lysate with it, run a gel, cut the band, mass spec, and find out which proteins bind to your specific RNA of interest?

How could one make a bead with RNA on it? Is there a kit for that?

Thanks!




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