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protease inhibition


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#1 Silas Bruun

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Posted 26 February 2002 - 03:52 AM

Hi All
Im trying to purify recombinant protein from Coli cells (invitrogen one-shot cells). But during lysis of the cells my protein seem to be degraded. How can I prevent this?

#2 sean

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Posted 13 March 2002 - 05:49 PM

there are comercially available protease inhibitors, such as benzamidine or PMSF.  

There are other protease inhibitor mixes you can purchase also.

that should decrease amount of degradation you get.






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