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Why my micorRNA Knockdown can't be repeated?


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#1 littlecell

littlecell

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Posted 17 September 2010 - 08:30 AM

I have tried to overexpress the interested microRNAs using the plemir vector from Open Biosystem. I did see very good knockdown (at least 70%) of at least one target for each MicroRNA by western blot. But the problem is that the knockdown at the protein level is not reproducible at all even the same cell line was infected with the microRNA virus. I have been seeing this inconsistent western blot result for at least 2 different micrRNA constructs.
Does any of you has the same experience when you using Plemir from Open Biosystem?
Appreciate your suggestions and advice.




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