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NaDOC

Started by ragno, Mar 14 2002 09:09 AM

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#1 ragno

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Posted 14 March 2002 - 09:09 AM

Hello,
I would like to know the role of sodium deoxycholate about protein precipitation with TCA.
Thanks
michel

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#2 sean

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Posted 15 March 2002 - 04:16 PM

hey michel.

Na Deoxycholate is an ionic dtergent. ppl use it for getting memrbane proteins dissolved, etc.

depending on ur proteins and on when u wanna do ur ppt, it can be left out. ( at which evet u will ppt with tca/ethanol)

but i need to know what it is that u r doing.

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