Hello,
I would like to know the role of sodium deoxycholate about protein precipitation with TCA.
Thanks
michel
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sean
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Posted 15 March 2002 - 04:16 PM
hey michel.
Na Deoxycholate is an ionic dtergent. ppl use it for getting memrbane proteins dissolved, etc.
depending on ur proteins and on when u wanna do ur ppt, it can be left out. ( at which evet u will ppt with tca/ethanol)
but i need to know what it is that u r doing.
