I calibrate the qubit with these standards just fine. First I make up the Picogreen mix, then take 10ul of each standard and dilute into 190ul of Picogreen mix, so the standards are diluted 1/20. After qubit is calibrated, I measure the standards in the kit, they read as <0.50ng/ml (for the 0ng/ul tube) and >600ng/ml (for 10ng/ul tube).
My question is how does the qubit calculate the 10ng/ul labeled standard tube as being greater than 600ng/ml when it is diluted 20-fold. Can someone show me the calculation of how it does this?
Edited by molbio1234, 16 September 2010 - 06:51 PM.