I use the A280 absorbance to determine my His-tag purified protein. Just wondering if the A260/280 ratio means anything in this case? I have been getting 260/280~ 2.0. Does this mean there's nucleic acid contamination? What should I do??
I know in determining DNA concentration if A260/280 is low then that means there's probably protein or other substance contamination.
Protein A280 (260/280 ratio)?
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