3 replies to this topic

[Finding it out]

Hi All

I am trying to dissolve my flavonoids in dmso at a conc low enough to be not toxic to s.aureus. The problem is at a DMSO conc lower than 70% the flavonoid comes out of solution. Any suggestions?

P.S the flavonoid will not dissolve in any other solution

Thanks

[MJD]

what is the flavonoid? Has it been used previously? Check pubmed or google scholar - if so check the methods section usually it tells you what they used to dissolve the flavonoids.

Another idea is that if you can make a mM or M stock, then when you treat your cells you dilute it to uM concentrations.  I done this before with a compound I dissolved at 20mM, then I used a 200uM working stock and a range between 2-20uM for treatment concentrations.

Edited by MJD, 16 September 2010 - 09:54 AM.

[Liu Yao]

Finding it out, on 16 September 2010 - 08:23 AM, said:

Hi All

I am trying to dissolve my flavonoids in dmso at a conc low enough to be not toxic to s.aureus. The problem is at a DMSO conc lower than 70% the flavonoid comes out of solution. Any suggestions?

P.S the flavonoid will not dissolve in any other solution

Thanks

My suggestion is more or less the same, I don't know which flavonoid you are dealing with, but I had to work with quite some different flavonoids. They are very difficult to dissolve. What I did is to dissolve them in high conc. such as 100mM and then dilute them with the certain solution I use for my cell experiment when I need to use them. Some times I needed to do it in a 60 centidegrees water bath.

[DNA]

I also make 100 mM stock and then dilute them to get thE desired uM and nM concentrations.