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EC50, ED50?


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#1 ethan241

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Posted 15 September 2010 - 12:48 PM

I'm making a primary neuronal cell culture line and I want to put several different concentrations of morphine on it to see if I can very the results, how do I find the EC50 or ED50 for morphine> Is this a good place to start, then steadily increase until about 10uM?

Thanks in advance!

#2 nightingale

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Posted 16 September 2010 - 02:55 PM

hello ethan241,

well .. here i attach you a paper that shows you how to calculate the ED50 & the EC50 ...
wished i found a simpler one !

and here is a link to a paper computer programs for calculating LD50 or ED50

Best Wishes,
nightingale

Attached File  theoretical basis experimental design.pdf   1.86MB   4013 downloads
" The more you learn, the more you realize how little you know ... "




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