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lentivirus for invitro study


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#1 satty

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Posted 15 September 2010 - 12:05 PM

Hi

Does anyone know if i can use the media supernatant of my lentivirus for infecting cells (in vitro experiments) or i have to follow the complete protocol of purifying and concentrating the virus and then using on cells...i know for in vivo studies i need the virus to be pure and conc. but is it required for the in vitro stuff too????

#2 laurequillo

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Posted 15 September 2010 - 12:06 PM

Hi

Does anyone know if i can use the media supernatant of my lentivirus for infecting cells (in vitro experiments) or i have to follow the complete protocol of purifying and concentrating the virus and then using on cells...i know for in vivo studies i need the virus to be pure and conc. but is it required for the in vitro stuff too????


Yes, you can use it directly
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