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SDS-PAGE for proteins which have pI>7 - SDS-PAGE and Western Blotting - BioForum

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SDS-PAGE for proteins which have pI>7


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#1 bfishie

bfishie

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Neutral

Posted 15 September 2010 - 09:27 AM

Hi all,

I have several proteins which have pI>7. So, at neutral pH values and room temperature, I get precipitates. Thus, I dissolved my proteins in 6 GHCl to prevent precipitation and lowered the pH. But now, I cannot run them on gel, they remain attached to the wells. I am looking for a suitable protocol.

Also I came across several native PAGE procedures, but I want to perform native PAGE, since I know I have several oligomeric states. That might be the next step for me.

Thanks!

#2 mdfenko

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Excellent

Posted 17 September 2010 - 10:32 AM

your title is misleading. it says sds-page.

you can use acid-page.
talent does what it can
genius does what it must
i do what i get paid to do




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