Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Maize embryogenic cell culturing help


  • Please log in to reply
No replies to this topic

#1 DavidUK

DavidUK

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 15 September 2010 - 06:23 AM

Hi,

I have recently started to try and produce a cell culture using maize embryo's, initially on solid media in order to gain the callus structures that I need. The problem that I am having is that all the embryo's that I extract and place onto plates for growth seem to then just enlarge and pretty much germinate.

I don't know if this is because the embryo's used for this were too far along developmentally or a different problem. I am aware that there are larger more solid embryo's and then also slightly smaller translucent ones.


- Embryo's used between 1-2mm in length
- Plates are kept in the dark at 25.C
- Using MS media, agar, 2mg/l 2,4-D and MS vitamin addition in plate media.

As far as I know the everything up to placing the embryo onto the plate is fine (extraction, sterilization, embryo axis on media, etc) therefore the only thing I can think of is either something to do with the media composition/the way i have made it or the embryo stage I am using.

I have yet to see anything that looks like a callus of any sort in the images that I have seen, just enlarging embryo's.

(I have probably left out quite a lot of details)
Any help would be greatly appreciated :)

Dave




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.