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HT1080 karyotype


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#1 aminoxy

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Posted 14 September 2010 - 12:03 PM

Hi everyone,

how many copies of Chromosome 7 does the HT1080 cell line contain? I've asked ATCC, but they seem to be totally ignorant.
Is there a directory with the expected karyotype of such a cell line?

Your help is highly appreciated.

Thanks!

#2 Anitachrom

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Posted 21 December 2010 - 12:05 PM

Hi, if you want to know the karyotype of the HT-1080 grown in your lab, you'll need to have a metaphase prep made and have a cytogenetics lab look at it for you. Though there is a "standard" karyotype for HT-1080, like any long-in-culture line, the karyotype changes over time, and sublines may develop. Doubling of chromosome number from diploid to tetraploid is not uncommon. Keep in mind also that a karyotype including "-7" doesn't mean there is only one copy of 7, it means there is only one NORMAL 7 present. Some or all of the missing 7 may be present in other abnormal ("marker") chromosomes. FISH may be need to determine what chromosome 7 material is present, and where.

Don't blame the ATCC, complete karyotype info on all the cell lines they have available is a herculean (and expensive)task, and one they have pretty much abandoned.

Yes, there are cell line databases, but many do not include karyotype, or like the ATCC, the information is out-of-date. Do some seraching, also check the literature for the line and its karyotype (remember to try it with and without the - in the name).

Good luck to you!




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